Chromatography of Lipids

Thin-layer Chromatography of Lipids

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...

Chromatography of unusual lipids on thin layers of magnesium oxide.

The chromatographic behavior of some minor components of natural lipids was studied using layers of magnesium oxide and of commercial adsorbents containing magnesium oxide. The lipids investigated included wax esters, cholesteryl esters, diester waxes, and esters, ethers and ether-esters of ethanediol and glycerol. Pronounced differences were found in the patterns of separation of certain lipid...

Chromatography of Lipids on Commercial Silica Gel Loaded Filter Paper.

Separation of lipids by Silica Gel G column chromatography.

A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method afford...

Thin-layer chromatography of tissue lipids without extraction.

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.